High Resolution (nano-)Optics [中 文]

Introduction: Bio-nanotechnology is an area of great research interest. Fundamental to research in this area is the technique of single molecule detection � the ultimate limit in nanotechnology. In contrast to conventional technology, the art of observing single molecules requires new techniques in order to limit the generation and collection of background noise while maximizing the collection of signal. Only in 1993 were single dye molecules were observed at room temperature [1] utilizing near-field optics and only in 1996 were single molecules observed using far-field techniques[2]. In the past, we combined a discarded AFM stage and a conventional microscope to build a cost effective confocal microscope. Unfortunately, the environment greatly affects lifetime.

Short term objectives: We propose to develop and apply a novel confocal microscope that overcomes three key problems associated with current practices.

  1. Environmental control
  2. High NA collection
  3. High-speed data acquisition.

Long term objectives: Utilization of this microscope:

  1. To explore the effect of environment on the photophysics of luminescent conjugated polymers answering whether humidity or oxygen is more detrimental to polymer lifetime and exploring the fluorescence quenching mechanism in conjugated polymers (hopefully in collaboration with Fann Wunshain (NTU, Dept. of Physics)).
  2. To investigate drug entry into cells (in collaboration with Prof. Zhang King Jen at NTUH).

Research Plan: (Click on the diagram)

Next steps (available Master's Projects):

[1] E. Betzig, R. J. Chichester, Science 262, 1422 (1993)
[2] J. J. Macklin et al, Science 272, 255 (1996)

© Jonathon David White
- "With a Servant's Heart" -
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